General ligands and (Co)substrate elution in affinity chromatography
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چکیده
منابع مشابه
Purification of glycolytic enzymes by using affinity-elution chromatography.
1. A systematic procedure for the purification of enzymes by affinity-elution chromatography is described. Enzymes are adsorbed on a cation-exchanger, and eluted with ligands specific for the enzyme concerned. 2. All of the glycolytic and some related enzymes present in rabbit muscle can be purified by the affinity-elution technique. The pH range for adsorption and elution of each enzyme was fo...
متن کاملPrediction of neutral salt elution profiles for affinity chromatography.
Neutral salts exhibit very marked differences as eluants of proteins from affinity columns. We observe: (i) that the relative potencies of neutral salts as eluants are independent of the protein or the affinity ligand in the systems studied, (ii) that the absolute salt concentration necessary to elute any given protein bound to the affinity matrix is proportional to the algebraic sum of a set o...
متن کاملPDZ affinity chromatography: a general method for affinity purification of proteins based on PDZ domains and their ligands.
PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ∼90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholipids. We hypothesized that the specific, strong interactions of PDZ domains with their ligands woul...
متن کاملBiospecific Elution from Non-Specific Adsorbents (‘Affinity Elution’)
Enzymes adsorbed to ion exchangers are in many cases specifically desorbed by interaction with their ligands. This phenomenon has been variously called ‘specific elution with substrate’ or ‘affinity elution’ in the literature (von der Haar, 1974). Because the most effective ligands for elution are those containing charged groups identical with those on the ion exchanger (positive groups in the ...
متن کاملLarge-Scale Gradient Elution Chromatography
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1270012p